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Image Search Results
Journal: eLife
Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy
doi: 10.7554/eLife.35755
Figure Lengend Snippet: ( A ) Confocal images showing cell surface expression in mammalian HEK293 cells of 5-HT receptors cloned from S. mansoni ( Sm .5HTR L ), S. haematobium ( Sh. 5HTR) and S. japonicum ( Sj .5HTR) localized by COOH-terminally tagged eGFP. Scalebar, 50 µm. ( B ) Schematic of luminescent cAMP sensor bioassay. cAMP generated by schistosome 5-HTRs (blue) binds the engineered GloSensor luciferase, switching the sensor to a more active conformation resulting in enhanced luminescence output. ( C ) Kinetics of signal following the addition of 5-HT (1 µM) to HEK293 cells co-transfected with luminescent cAMP sensor and individual schistosome 5-HT receptors. Open circles, cells not transfected with 5-HT receptor, colored circles represent measurements in cells transfected with Sm .5HTR L (black), ( Sh. 5HTR (blue) and Sj .5HTR (red). ( D ) Serotonin dose-response curves for each of the three receptors (colored circles) and HEK293 cells expressing the cAMP sensor alone (open circles). Data reflect mean ± standard error of at least 3 biological replicates.
Article Snippet: Commercial assay or kit ,
Techniques: Expressing, Clone Assay, Bioassay, Generated, Luciferase, Transfection
Journal: eLife
Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy
doi: 10.7554/eLife.35755
Figure Lengend Snippet:
Article Snippet: Commercial assay or kit ,
Techniques: Recombinant, Software